Some eukaryotic proteins may simultaneously exist at, or move between, two or more subcellular locations. Proteins with multiple locations or dynamic features of this kind are particularly interesting because they may have some special biological functions intriguing to investigators in both basic research and drug discovery.To deal with these proteins, we have modified the ensemble classifier Euk-PLoc to cover cases of multiple locations as well. The modified classifier is called Euk-mPLoc.
Application scope: The prediction is for eukaryotic protein samples that covers the following 22 subcellular locations: (1) Acrosome; (2) Cell wall; (3) Centriole; (4) Chloroplast; (5) Cyanelle; (6) Cytoplasm; (7) Cytoskeleton; (8) Endoplasmic reticulum; (9) Endosome; (10) Extracell; (11) Golgi apparatus; (12) Hydrogenosome; (13) Lysosome; (14) Melanosome; (15) Microsome; (16) Mitochondrion; (17) Nucleus; (18) Peroxisome; (19) Plasma membrane; (20) Spindle pole body; (21) Synapse; and (22) Vacuole (see the figure below).

Stop prediction if the query protein is known not a eukaryotic protein or not one of the above 22 locations, because the result obtained will not make any sense.