Application scope: The prediction is for Gram-negative bacteria protein samples only that covers the following 8 subcellular locations: (1) cytoplasm, (2) extracellular, (3) fimbrium, (4) flagellar, (5) inner membrane, (6) nucleoid, (7) outer membrane, (8) periplasm (see the Figure below).

Stop prediction if the query protein is known not a Gram-negative protein or not one of the above 8 locations, because the result obtained will not make any sense.