Caveat

EzyPred is a top-down approach for predicting enzyme functional classes and sub-classes purely based on protein sequences. It consists of 3-layer prediction engines. The 1st layer predicts a query protein as enzyme or non-enzyme; if it is an enzyme, the 2nd layer will automatically predict which enzyme main-functional class it belongs to; subsequently, the 3rd layer will predict its sub-family class. The coverage scope of EzyPred is as follows.   1st layer   Enzyme protein   non-Enzyme protein   2nd & 3rd layer   1.-.-.- Oxidoreductases         1.1.-.- Acting on the CH-OH group of donors         1.2.-.- Acting on the aldehyde or oxo group of donors         1.3.-.- Acting on the CH-CH group of donors         1.4.-.- Acting on the CH-NH(2) group of donors         1.5.-.- Acting on the CH-NH group of donors         1.6.-.- Acting on NADH or NADPH         1.7.-.- Acting on other nitrogenous compounds as donors         1.8.-.- Acting on a sulfur group of donors         1.9.-.- Acting on a heme group of donors         1.10.-.- Acting on diphenols and related substances as donors         1.11.-.- Acting on a peroxide as acceptor (peroxidases)         1.12.-.- Acting on hydrogen as donor         1.13.-.- Acting on single donors with incorporation of molecular oxygen         1.14.-.- Acting on paired donors, with incorporation or reduction of molecular oxygen         1.15.-.- Acting on superoxide as acceptor         1.16.-.- Oxidizing metal ions         1.17.-.- Acting on CH or CH(2) groups         1.18.-.- Acting on iron-sulfur   2.-.-.- Transferases         2.1.-.- Transferring one-carbon groups         2.2.-.- Transferring aldehyde or ketone residues         2.3.-.- Acyltransferases         2.4.-.- Glycosyltransferases         2.5.-.- Transferring alkyl or aryl groups, other than methyl groups         2.6.-.- Transferring nitrogenous groups         2.7.-.- Transferring phosphorous-containing groups         2.8.-.- Transferring sulfur-containing groups   3.-.-.- Hydrolases         3.1.-.- Acting on ester bonds         3.2.-.- Glycosylases         3.4.-.- Acting on peptide bonds (peptide hydrolases)         3.5.-.- Acting on carbon-nitrogen bonds, other than peptide bonds         3.6.-.- Acting on acid anhydrides   4.-.-.- Lyases         4.1.-.- Carbon-carbon lyases         4.2.-.- Carbon-oxygen lyases         4.3.-.- Carbon-nitrogen lyases         4.4.-.- Carbon-sulfur lyases         4.6.-.- Phosphorus-oxygen lyases         4.99.-.- Other lyases   5.-.-.- Isomerases         5.1.-.- Racemases and epimerases         5.2.-.- Cis-trans-isomerases         5.3.-.- Intramolecular oxidoreductases         5.4.-.- Intramolecular transferases (mutases)         5.5.-.- Intramolecular lyases         5.99.-.- Other isomerases   6.-.-.- Ligases         6.1.-.- Forming carbon-oxygen bonds         6.2.-.- Forming carbon-sulfur bonds         6.3.-.- Forming carbon-nitrogen bonds         6.4.-.- Forming carbon-carbon bonds         6.5.-.- Forming phosphoric ester bonds         6.6.-.- Forming nitrogen-metal bonds (See the Figure 1 below)

Figure 1. A tree-branch illustration to show the coverage scope by the current EzyPred.